EK282HSS

Mouse TNF-α High Sensitivity Standard (小鼠TNF-α高敏 标准品)

¥180.00

  • 分子靶点：TNF, TNFA, TNFSF2
  • 种属：小鼠 (Mouse)
  • 试剂盒：EK282HS
  • 保存：短期4℃保存，长期-20℃保存
  • 运输条件：4℃蓝冰运输

在售SKU:70-EK282HSS

描述

本产品只包含标准品试剂，如需购买试剂盒请点击下图

商品名 Mouse TNF-α High Sensitivity Standard (小鼠TNF-α高敏 标准品)
组分 小鼠TNF-α高敏标准品
检测方法 双抗夹心法
样本类型 血清，血浆，细胞培养上清及其他生物学样本
板式 管
保存 短期4℃，长期-20℃保存
运输条件 4℃蓝冰运输

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠TNF-α抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的TNF-α与固相抗体结合。洗涤去除未结合的物质后，加入生物素化的检测抗体孵育。洗涤去除未结合的生物素化的抗体，加入辣根过氧化物酶标记的链霉亲和素 (Streptavidin-HRP)。洗涤后，加入信号增强剂孵育，洗涤去除未结合的物质后，再次加入Streptavidin-HRP。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中TNF-α的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse TNF-α High Sensitivity ELISA Kit (小鼠TNF-α高敏 ELISA试剂盒) - 标准曲线
标准曲线

分子信息

概述小鼠 TNF, TNFA, TNFSF2 靶点信息

TNF 分子靶点信息概述

  • 分子名：TNF, tumor necrosis factor
  • 基因家族：Tumor necrosis factor superfamily
  • 别名：TNFSF2; DIF; TNF-alpha
  • 曾用名：TNFA
  • 全称：TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2)

TNF 分子靶点综述

TNF-α 为单核细胞、巨噬细胞、中性粒细胞、NK 细胞和 CD4+T 细胞产生的 17 kDa 细胞因子。它在免疫应答中具有多种调节功能，还可作为潜在的热原。TNF-α对刺激物(感染因子或组织受损)产生应答后，在全身循环，激活中性粒细胞，改变血管内皮细胞的特性，调控其它组织的代谢活性，以及通过诱导局部凝血作用展现肿瘤杀伤活性。TNF-α 在关节组织和其它组织发生炎症的发病机制具有重要作用。

小鼠 Mouse Tnf 分子靶点信息

小鼠 Mouse Tnf 靶点分子功能（预测）

Enables cytokine activity; protease binding activity; and tumor necrosis factor receptor binding activity. Involved in several processes, including endothelial cell apoptotic process; positive regulation of cell communication; and positive regulation of macromolecule metabolic process. Acts upstream of or within several processes, including apoptotic signaling pathway; positive regulation of intracellular signal transduction; and regulation of gene expression. Located in several cellular components, including external side of plasma membrane; phagocytic cup; and recycling endosome. Is expressed in several structures, including alimentary system; brain; integumental system; lung; and reproductive system. Used to study several diseases, including autoimmune disease (multiple); congestive heart failure; heart valve disease (multiple); idiopathic pulmonary fibrosis; and spondyloarthropathy. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); lung disease (multiple); and skin disease (multiple). Orthologous to human TNF (tumor necrosis factor).

引用文献统计

该产品被引用的文献总数为：1

ELISA标准品操作常见问题

小心！你的 ELISA 试剂盒可能是假的

小心！你的 ELISA 试剂盒可能是假的 

开学学习计划，如何收集ELISA检测样本——细胞

开学学习计划，如何收集ELISA检测样本——细胞 

开学学习计划，如何选择ELISA试剂盒

开学学习计划，如何选择ELISA试剂盒 

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开学学习计划，ELISA从新手到入门！ 

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ELISA通关必备丨数据篇丨标准曲线不佳

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒

ELISA通关必备丨如何选择试剂盒 

查看更多ELISA标准品操作步骤技术文章

检测步骤

ELISA操作常见问题

小心！你的 ELISA 试剂盒可能是假的 

开学学习计划，如何收集ELISA检测样本——细胞 

开学学习计划，如何选择ELISA试剂盒 

开学学习计划，ELISA从新手到入门！ 

一文掌握ELISA实验显色判断、数据分析及标曲拟合 

血清OR血浆，哪个是ELISA的菜 

ELISA通关必备丨数据篇丨标准曲线不佳 

ELISA通关必备丨操作篇丨常见问题及解决方案 

ELISA通关必备丨操作篇丨溶解与稀释标准品 

ELISA通关必备丨样本篇丨不常见样本 

ELISA通关必备丨样本篇丨常见样本丨细胞 

ELISA通关必备丨如何选择试剂盒 

ELISA通关必备丨基础知识 

真？假？ELISA试剂盒选择要小心 

ELISA常见类型一 | 双抗夹心法，你要的都在这里！ 

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引用文献

文章目录[隐藏]


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  8. Propionate alleviated post-infarction cardiac dysfunction by macrophage polarization in a rat model 

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  12. A network pharmacology strategy combined with in vitro experiments to investigate the potential anti-inflammatory mechanism of Prunus cerasifera Ehrhart 

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  34. Tanreqing Inhibits LPS-Induced Acute Lung Injury In Vivo and In Vitro Through Downregulating STING Signaling Pathway 

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  36. Gastroprotective effect and mechanisms of Chinese sumac fruits (Rhus chinensis Mill.) on ethanol-induced gastric ulcers in mice 

  37. LncRNA Neat1/miR-298-5p/Srpk1 Contributes to Sevoflurane-Induced Neurotoxicity 

  38. Anti-B7-H3 monoclonal antibody ameliorates the damage of acute experimental pancreatitis by attenuating the inflammatory response 

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  41. Chemical constituents and anti-inflammatory activity of the total alkaloid extract from Melodinus cochinchinensis (Lour.) Merr. and its inhibition of the NF-κB and MAPK signaling pathways 

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  43. Chronic exposure to microcystin-leucine-arginine induces epithelial hyperplasia and inflammation in the mouse bladder 

  44. Chromium alters lipopolysaccharide-induced inflammatory responses both in vivo and in vitro 

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  66. Huangshan Floral Mushroom Polysaccharide Ameliorates Dextran Sulfate Sodium-Induced Colitis in Mice by Modulating Th17/Treg Balance in a Gut Microbiota-Dependent Manner 

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  78. Nauclea officinalis inhibits inflammation in LPS-mediated RAW 264.7 macrophages by suppressing the NF-κB signaling pathway 

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  83. Protective effect of cynaroside on sepsis-induced multiple organ injury through Nrf2/HO-1-dependent macrophage polarization 

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  118. A molybdenum oxide-based degradable nanosheet for combined chemo-photothermal therapy to improve tumor immunosuppression and suppress distant tumors and lung metastases 

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